Plasmid

Part:BBa_K1391006:Design

Designed by: Alexa Garcia   Group: iGEM14_MIT   (2014-10-16)


Syk


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1934
    Illegal PstI site found at 442
    Illegal PstI site found at 1022
    Illegal PstI site found at 1811
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1934
    Illegal PstI site found at 442
    Illegal PstI site found at 1022
    Illegal PstI site found at 1811
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1509
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1934
    Illegal PstI site found at 442
    Illegal PstI site found at 1022
    Illegal PstI site found at 1811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1934
    Illegal PstI site found at 442
    Illegal PstI site found at 1022
    Illegal PstI site found at 1811
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Human genome

References